TITLE:
Effects of MTG and GSH on Human Sperm Motility and DNA Integrity during Vitrification in the Presence of Trehalose
AUTHORS:
Xiaowei Shi, Huanhuan Hu, Guojie Ji, Rui Liu, Jing Zhang, Han Zhang, Pengbo Wang, Bingxin Qin, Bingjie Li, Mingwen Li
KEYWORDS:
Human, Sperm, Vitrification, Trehalose, Antioxidant
JOURNAL NAME:
Advances in Reproductive Sciences,
Vol.8 No.1,
February
14,
2020
ABSTRACT: Limited information exists about the effects of antioxidants on sperm motility and DNA integrity during vitrification in humans. This study compared the effects of reduced glutathione (GSH) and monothioglycerol (MTG) at different concentrations on post-thaw sperm motility and DNA integrity after vitrification in humans using 0.25 M trehalose as a cryoprotective agent, and found that supplementation of MTG or GSH at 0.5 mM resulted in significantly higher (P