TITLE:
Effect of H2O2-Mediated Endophytic Fungal Elicitors on Essential Oil Accumulation in Suspension Cells of Cinnamomum longepaniculatum
AUTHORS:
Kuan Yan, Yu Zheng, Lan Hu
KEYWORDS:
Endophytic Fungal Elicitor, H2O2, 1, 8-Eucalyptus, Signal Transduction, CAT
JOURNAL NAME:
Open Access Library Journal,
Vol.7 No.1,
January
14,
2020
ABSTRACT: Hydrogen peroxide (H2O2) is a signal molecule that plays a crucial role in plant secondary metabolism. In order to explore the signaling mechanism of endophytic fungal elicitors (Penicillium commune 2J1) for promoting 1,8-eucalyptus accumulation in C. longepaniculatum, changes in the contents of H2O2 and 1,8-eucalyptus were investigated after the addition of elicitors to the C. longepaniculatum cultures. The experimental results showed that the 1,8-eucalyptus contents in C. longepaniculatum cells were increased upon addition of the endophytic fungal elicitors into the culture. It’s maybe through Hydrogen peroxide (H2O2) signal molecule. When different concentrations of elicitors were added to C. longepaniculatum suspension cells, the changes in the content of H2O2 and 1,8-eucalyptus showed the same trend, and both reached the highest value at 40 mg/L of elicitor concentration, indicating that the endogenous fungal elicitors affect the accumulation of 1,8-eucalyptus through H2O2 signaling molecular pathway. When CAT was added, the 1,8-eucalyptus decreased but was not completely inhibited, indicating that the elicitors also have other signaling pathways.