TITLE:
Potential Antidiabetic Activities of Probiotic Strains, L. acidophilus and L. bulgaricus against Fructose-Fed Hyperglycemic Rats
AUTHORS:
Md Shihab Uddin Sohag, Mollika Paul, Md Abdul Alim Al-Bari, Mir Imam Ibne Wahed, Md Rafiqul Islam Khan
KEYWORDS:
Probiotics, Fructose-Fed Rats, Hyperglycemia, Dyslipidemia
JOURNAL NAME:
Food and Nutrition Sciences,
Vol.10 No.12,
December
30,
2019
ABSTRACT: Background: Deregulation of the gut microbiota results in various pathological disorders such as diabetes, inflammation, cancer,
dyslipidemia etc. Modulation of intestinal microbiota by probiotics may
facilitate the management of a number of clinical conditions of diabetes. Methods: The present study was designed to investigate the effect of feeding low-fat
probiotic yogurt containing L. acidophilus and L. bulgaricus on
fructose-fed hyperglycemic rats. Yogurt
containing L. acidophilus or L. bulgaricus (9.5 × 109 cfu/rat/day) alone or in combination of both strains was supplied
orally for 8 weeks concurrently with 20% fructose solution. Fasting blood
glucose (FBG), oral glucose tolerance test, glycosylated hemoglobin (HbA1c),
lipid profiles from blood and histopathological study of liver tissues were
analyzed to evaluate anti-diabetic effect.
Statistical analysis was done by Graph Pad Prism software. Values at p 0.05 were considered statistically significant. Results: Administration
of L. acidophilus or L. bulgaricus alone or in combination of
both to hyperglycemic rats decreased serum FBG, onset of glucose intolerance, HbA1c, total
cholesterol, triglycerides, LDL and VLDL-cholesterol, increased HDL-cholesterol levels significantly and preserved
antioxidant pool such as activities of superoxide dismutase, catalase etc.
Probiotic administration also prevented/repaired the oxidative damage of liver tissues. Conclusion: In conclusion,
administration of yogurt containing L. acidophilus or L. bulgaricus that balanced the intestinal
microbiota can prevent or lower risks of type-2 diabetes
and its related complications.