TITLE:
Structure Prediction and Enzymatic Properties of Phytase phyS
AUTHORS:
Yueming Li, Hongqing Xu, Jianchun Xu, Ruilian Pang, Bingzheng Xu
KEYWORDS:
Structural Prediction, Enzymatic Properties, Phytase
JOURNAL NAME:
Advances in Enzyme Research,
Vol.7 No.4,
December
24,
2019
ABSTRACT: Phytase is a kind of enzyme that hydrolyzes phytic acid and its salts to produce inositol and phosphoric acid. As a new feed additive, phytase has great potential in animal nutrition and environmental protection. Because of its good stability, large-scale production and high activity, microbial phytase has become a hot spot in industrial application. Here, we reported the predicted structure and enzymatic properties of a phytase from Bacillus subtilis, which was named as phyS. It was clear that the optimal temperature is 35°C, and the optimal pH is 8. Meanwhile, the enzyme activity was kept at above 90% in the range of pH 8 - 9, this result demonstrated that phyS is an alkaline phytase. This study lays a foundation for the extensive application of phyS.