TITLE:
Flow Injection Determination of Tramadol Based on Its Sensitizing Effect on the Chemiluminescent Reaction of Permanganate-Sulfite
AUTHORS:
Xun Yao, Jingkai Zhang, Jianguo Li
KEYWORDS:
Chemiluminescence, Tramadol hydrochloride, KMnO4, Sodium Sulfite, Flow-Injection Analysis
JOURNAL NAME:
American Journal of Analytical Chemistry,
Vol.2 No.7,
October
31,
2011
ABSTRACT: In this paper, a novel chemiluminescence (CL) method for the determination of tramadol has been developed by combining the flow injection technique and its sensitizing effect on the weak CL reaction between sulfite and acidic KMnO4. A mechanism for the CL reaction has been proposed on the basis of fluorescent and CL spectra. Under the optimized conditions, the proposed method allows the measurement of tramadol hydrochloride over the range of 0.04 - 4 ?g/mLwith a correlation coefficient of 0.9995 (n = 8) and a detection limit of 0.01 ?g/mL (3σ), and the relative standard deviation for 2.0 ?g/mL tramadol (n = 11) is 2.1%. The utility of this method was demonstrated by determining tramadol hydrochloride in tablets and injections.