Article citationsMore>>

Silva, M.J., Sousa, L.M., Lara, V.P., Cardoso, F.P., Júnior, G.M., Totola, A.H., Caliari, M.V., Romero, O.B., Silva, G.A., Ribeiro-Sobrinho, A.P. and Vieira, L.Q. (2011) The Role of iNOS and PHOX in Periapical Bone Resorption. Journal of Dental Research, 90, 495-500.
https://doi.org/10.1177/0022034510391792

has been cited by the following article:

• TITLE: Effect of Colchicine on Inducible Nitric Oxide Synthase Activity and Nitric Oxide Production of Mice Induced by Aggregatibacter actinomycetemcomitans

  AUTHORS: Wihaskoro Sosroseno, Endang Herminajeng, Phillip S. Bird

  KEYWORDS: Aggregatibacter actinomycetemcomitans, Colchicine, NO, iNOS, Mice

  JOURNAL NAME: Journal of Biosciences and Medicines, Vol.7 No.12, December 2, 2019

  ABSTRACT: Objective: Colchicine induced a non-protective Th2-like immunity in Aggregatibacter actinomycetemcomitans-stimulated murine immune response. The aim of the present study was to determine whether colchicine affects inducible nitric oxide synthase (iNOS) activity and nitric oxide (NO) production in A. actinomycetemcomitans-immunized mice. Materials and Methods: BALB/c mice were sham-immunized (group I) or immunized with heat-killed A. actinomycetemcomitans (group II-VII). Colchicine was injected intraperitoneally before (group III), on the same day of (group IV), or after (group V) the primary immunization and on the same day of (group VI) or after (group VII) the secondary immunization. In vitro, spleen cells from either sham- or heat-killed A. actinomycetemcomitan-immunized animals were cultured and stimulated with heat-killed A. actinomycetemcomitans in the presence or absence of colchicine with or without addition of L-arginine, Db-cAMP, forskolin or interferon-γ (IFN-γ). The levels of splenic iNOS activity and both serum and culture supernatant NO levels were assessed. Results: The results showed that colchicine did inhibit both splenic iNOS activity and serum NO levels only when the drug was injected at the same time as the immunization (group IV and VI). Splenic iNOS activity and NO levels on antigen-stimulated spleen cell cultures were also suppressed by colchicine, even in the presence of L-arginine, Db-AMP or forskolin. IFN-γ only partially restored iNOS activity and NO levels in the antigen and colchicine-treated spleen cell cultures. Conclusion: This study suggests, therefore, that colchicine may suppress the iNOS activity and NO production in A. actinomycetemcomitans-immunized mice in vivo and in vitro.