TITLE:
Targeted Genome Engineering and Its Application in Trait Improvement of Crop Plants
AUTHORS:
Xiaoyu Yang, Linlin Luo, Beixin Mo, Lin Liu
KEYWORDS:
Targeted Genome Engineering, Site-Specific Recombination, ZFN, TALEN, CRISPR/Cas9, Crop Improvement
JOURNAL NAME:
Agricultural Sciences,
Vol.10 No.10,
October
18,
2019
ABSTRACT: Targeted genome engineering refers to technologies that are used for site-specific genome modifications such as knockout, knockin and transcriptional regulation of genes of interest in organisms. Site-specific recombination system, zinc finger nucleases (ZFNs), transcriptional activator-like effector nucleases (TALENs) and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein-9 nuclease (Cas9) (CRISPR/Cas9) technologies are the representatives of targeted genome engineering and have been widely used in crop basic and applied research. In this review, we introduce the basic information and action modes of these different genome engineering technologies, summarize the recent progresses of targeted genome engineering technologies and their applications in crop improvement, and propose perspectives for genome engineering-mediated modifications of crop plants in the future.