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Den Hartigh, L.J., Omer, M., Goodspeed, L., Wang, S., Wietecha, T., O’Brien, K.D. and Han, C.Y. (2017) Adipocyte-Specific Deficiency of NADPH Oxidase 4 Delays the Onset of Insulin Resistance and Attenuates Adipose Tissue Inflammation in Obesity. Arteriosclerosis, Thrombosis, and Vascular Biology, 37, 466-475.
https://doi.org/10.1161/ATVBAHA.116.308749

has been cited by the following article:

• TITLE: Tephrosia purpurea Fraction Attenuates Lipid Accumulation and Adipogenesis in 3T3-L1 Adipocytes and Reduces Body Weight in High Fat Diet Induced Obese Rats

  AUTHORS: Karunakaran Reddy Sankaran, Lokanatha Oruganti, Krishna Chintakuntla, Balaji Meriga

  KEYWORDS: Body Weight, Cell Viability, Enzyme Inhibition, Lipolysis, 3T3-L1 Cells

  JOURNAL NAME: Advances in Bioscience and Biotechnology, Vol.10 No.9, September 30, 2019

  ABSTRACT: The anti-adipogenic and anti-obesity activity of chloroform fraction of Tephrosia purpurea (CFTp) on 3T3-L1 adipocytes and high fat diet (HFD)-fed obese rats was evaluated in this study. A substantial and dose dependent inhibition of α-glucosidase (81%) and lipase (75%) activities by CFTp was noticed. Treatment with CFTp (250 μg/mL) significantly inhibited 3T3-L1 adipocytes differentiation and lipid accumulation. A semi-quantitative RT-PCR analysis of 3T3-L1 cells revealed down regulation of mRNA expression of peroxisome proliferator-activated receptor-γ (PPAR-γ), fatty acid synthase (FAS) and acetyl CoA carboxylase-2 (ACC-2), while glucose transporter type-4 (GLUT-4) expression was up-regulated in a dose dependent manner with CFTp. Further, oral administration of CFTp (200 mg/kg.b.wt.) significantly reduced body weight gain, fat mass, blood glucose and leptin levels in high fat diet (HFD)-induced obese rats. Taken together, these findings demonstrate that CFTp possesses potent anti-obesity activities.