TITLE:
Catechins Green Tea GMB4 Clone Increases mRNA of ABCA1 through LXR Signaling in Cultured Macrophage Exposed OX-LDL
AUTHORS:
Erna Susanti, Retty Ratnawati, Aulanni’am Aulanni’am, Achmad Rudijanto
KEYWORDS:
Atherogenesis, Catechins, ABCA1, ABCG1 Dan SRB1, Cultured Macrophage
JOURNAL NAME:
Food and Nutrition Sciences,
Vol.10 No.9,
September
29,
2019
ABSTRACT: Inhibition of atherogenesis through inhibition of lipid metabolism has
not been explored while other inhibitions through inflammation, endothelial dysfunction and free radicals have done.
Inhibition of atherogenesis via inhibition of lipid metabolism can be done
through the mechanism of Reverse Cholesterol Transport (RCT). Signaling
pathways that play a role in this mechanism is LXR signaling. LXR activation by
an LXR agonist led increasing cholesterol efflux. Catechins based on
bioinformatics study showed as a potent candidate LXR agonists that can be used
as an inhibitor of atherogenesis. This study aims to prove that the
administration Catechins green tea Clone GMB4 can prevent atherosclerosis
through increasing mechanism cholesterol efflux from macrophage by taking
effect of ABCA1, ABCG1, SRB1 gene expression in cultured macrophages were exposed
ox-LDL. Long-term goals of the outcome of the research are the use of Catechins
Green Tea Clones GMB4 as an inhibitor of atherogenesis so that it can be used
as a complementary therapy for the treatment of atherosclerosis and
cardiovascular diseases. The research is divided into 5 groups, namely the culture of macrophages without exposed ox-LDL, culture exposed ox-LDL and groups of Catechins dose I, II, III. In vitro study showed that administration of Catechins increases mRNA of ABCA1,
whereas mRNA ABCG1 and SRB1 decreased at all three doses given. The result of
protein profilling was identified a protein with a molecular weight of 70 kDa
by SDS-PGE with silver staining.