TITLE:
Effects of Mitochondrial DNA Deletion and OSW-1 on PI3K-AKT Signaling Pathway PCR Array in SK-Hep1 Cells
AUTHORS:
Xichen Du, Changshi Qian, Xinglin Jin
KEYWORDS:
mtDNA, OSW-1, PI3K-AKT, PCR Array, SK-Hep1 Cells
JOURNAL NAME:
Chinese Medicine,
Vol.10 No.3,
September
26,
2019
ABSTRACT: To investigate the effect of mitochondrial DNA
deletion and OSW-1 on PI3K-AKT signaling pathway PCR Array in SK-Hep1
hepatocellular carcinoma cells, we prepared
SK-Hep1 cells with mtDNA deletion, that is, p0SK Hep. Then the OSW-1 of 100 ng/L was used to intervene SK-Hep1 and p0SK-Hep1.
RT-qPCR was used to detect the difference of gene expression on PI3K-AKT signaling pathway
PCR Array in four
groups of cells. The gene expression of TLR4, FOS and TSC2 markers in SK-Hep1 cells treated
with OSW-1 were significantly increased. The gene expressions of PDPK1, GJA1, TLR4 and TSC2 markers were significantly increased in p0SK-Hep1 cells, and the gene
expressions of IRAK1
and GJA1 markers were significantly increased in p0SK-Hep1 cells treated with
OSW-1. OSW-1 mainly affects the genes related to TLR4 pathway on PI3K-AKT
signaling pathway PCR Array in SK-Hep1 HCC cells. P0SK-Hep1 mainly
affects the upstream PDK1 gene and downstream TSC2 gene of Akt on PI3K-AKT signaling pathway PCR Array, and also
affects the gene expression of gap junction at the same time.