TITLE:
Molecular Characterization of a Large Diversity of Bambara Groundnut Potyviruses (Vigna subterranea (L.) Verdc.) in Burkina Faso
AUTHORS:
Aboubié Elisabeth Zongo, Bouma James Néya, Essowé Palanga, Drissa Sérémé, Moustapha Koala, Régis Dimitri Sokpé Longué, Jean Zabré, Zakaria Bouda, Nicolas Barro, Oumar Traoré
KEYWORDS:
Bambara Groundnut, Potyvirus, Characterization, Coat Protein Sequence, Diversity
JOURNAL NAME:
Advances in Bioscience and Biotechnology,
Vol.10 No.9,
September
24,
2019
ABSTRACT: Potyviruses are major constraints to grain legume production
by causing significant yield losses. Potyviruses infecting Bambara groundnut (Vigna subterranea)
were investigated in Burkina Faso. Leaf samples collected from three
agroclimatic zones were subjected to RT-PCR and sequence analyses. Of a total
of 135 samples, 36 (26.67%) were detected positive in RT-PCR tests using
potyvirus universal primers. Analysis of full coat protein (cp) sequences from
24 isolates revealed the occurrence of three groups of Bambara
groundnut-infecting potyviruses. Virus isolates in group 1 shared 94.5% - 100% nucleotide (nt)
identity with CABMV whereas those in group 2 and group 3 were distantly related Bean common necrosis virus (BCMNV)
and Passiflora virus Ugandan which
were their respective closest potyviruses. Group 2 shared 77.1% nt and 78.8% - 79.9% aa
identity with BCMNV and group 3 shared 77.3% - 78.3% nt and 80.7% - 81.5% aa
identity with Passiflora virus Ugandan.
All three groups were confirmed by phylogenetic analyses. Taking into account
potyvirus demarcation criteria, group 1 isolates belonged to CABMV species.
Group 2 and group 3 were assigned to a potentially new potyviruses species and
designated Bambara groundnut potyvirus 1 (BGPV1) and Bambara groundnut potyvirus 2 (BGPV2).