TITLE:
Comparison of Individual and Synergistic Antimicrobial Activity of Common Spices against Certain Infectious Pathogen in Bangladesh
AUTHORS:
Sohana Parvin Chowdhury, Avijit Banik, Shahrin Akter Aurin, Sabera Saima
KEYWORDS:
Spice, Antibacterial Activity, Ethanol Extract, MIC, MBC, Synergistic Effect
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.10 No.9,
September
19,
2019
ABSTRACT: Aims: The aim of this undertaken investigation was designed to determine the
comparative antimicrobial potential of ethanol extract of six commonly consumed spices such as
Garlic (Allium satilyvum), Ginger (Zingiber
officinale), Turmeric (Curcuma longa),
Cinnamon (Cinnamomum zeylanicum), Cumin (Cuminum cyminum) and Black cumin (Nigella sativa). Method: This study includes, the efficacy of individual and
synergistic effect of these extracts that was tested against bacteria by agar well-diffusion method
employing 100 μL spices-extract solution per well and was conducted in (Centre of Excellence Laboratory) Department of Microbiology, Primeasia University
during November 2018 to April 2019. Minimum inhibitory concentration (MIC) was determined by the micro-broth
dilution method and compared with commercial antibiotic discs such as
Amoxicillin, Vancomycin, Erythromycin, Ceftriaxone, Chloramphenicol, and
Ciprofloxacin. Result: According to the findings of the antibacterial
assay, the ethanol extracts of the spices showed inhibitory activity against
common infectious bacterial pathogens. Spice extracts have the most significant activity
against B. cereus and E. coli was the least sensitive among the tested organisms. The
ethanol extract had individual antibacterial activity with mean zone of
inhibition 22 ± 0.5 and 20.08 ± 0.58 mm and the synergistic effect of ethanol
extract had a mean zone of inhibition 30 ± 0.75 and
28.25 ± 0.9 mm against B. cereus and V. cholera, respectively, which is highly comparable to the
commercial antibiotic, Ciprofloxacin (25 mm). Conclusion: The ethanol extract of indigenous spices was shown
to be highly potential to be applied as an alternative of commercial drugs.