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Eyoh, A.B., Toukam, M., Atashili, J., Fokunang, C., Gonsu, H., Lyonga, E.E., Mandi, H., Ikomey, G., Mukwele, B., Mesembe, M. and Assoumou, M.C.O. (2014) Relationship between Multiple Drug Resistance and Biofilm Formation in S. aureus Isolated from Medical and Non-Medical Personnel in Yaounde, Cameroon. Pan African Medical Journal, 17, 186.
https://doi.org/10.11604/pamj.2014.17.186.2363

has been cited by the following article:

• TITLE: Molecular Detection of Biofilm-Producing Staphylococcus aureus Isolates from National Orthopaedic Hospital Dala, Kano State, Nigeria

  AUTHORS: U. Abdulrahim, M. Kachallah, M. Rabiu, N. A. Usman, G. O. Adeshina, B. O. Olayinka

  KEYWORDS: Staphylococcus aureus, Biofilm, Intercellular Adhesion and Adhesin Genes, Orthopaedic Patients, Microtitre Plate, Congo Red Agar

  JOURNAL NAME: Open Journal of Medical Microbiology, Vol.9 No.3, September 19, 2019

  ABSTRACT: This study evaluated biofilm formation and antibiotic susceptibility in 36 clinical S. aureus isolates recovered from orthopaedic patients and detected the presence of intercellular adhesion and adhesin genes. Staphylococcus aureus was isolated from nasal swab, wound and urine specimens collected from orthopaedic patients in National Orthopaedic Hospital Dala, Kano over a period of three months. The isolates were identified using rapid identification kit for Staphylococcus species. The antibiotics susceptibility of the isolates was determined using modified disc diffusion method. Phenotypically, the biofilm formation was assessed using the Congo red agar method and microtitre plate assay. Polymerase chain reaction (PCR) analysis was used to detect biofilm-associated genes and characterize the isolates. The isolation rate of S. aureus from the samples (n = 134) was 26.8%, mainly from nasal swab (36%) and wound swab (36%). A total of 19 (52.7%) of the isolates showed positive for slime production. Majority of the isolates 29/36 (81.6%) were biofilm positive with only 2 (5.5%) and 5 (13.8%) as strong biofilm-formers and moderate biofilm-formers respectively. Molecular evaluation of the biofilm-associated genes in 12 S. aureus isolates revealed the prevalence of bbp genes (25%), clfA genes (16.6%) and the icaA (8.3%). None of the isolates harboured the fnbA and cna genes. There is no significant difference (P > 0.05) in the antibiotic resistance pattern between biofilm-positive and biofilm-negative S. aureus isolates. This result revealed that phenotypically most of the S. aureus isolates were biofilm formers but few of them chromosomally harbour the biofilm-associated genes.