TITLE:
Effect of Macronutrients, Cytokinins and Auxins, on in Vitro Organogenesis of Thymus vulgaris L.
AUTHORS:
Zineb Nejjar El Ansari, Amina El Mihyaoui, Ibtissam Boussaoudi, Rajae Benkaddour, Ouafaa Hamdoun, Houda Tahiri, Alain Badoc, Aicha El Oualkadi, Ahmed Lamarti
KEYWORDS:
Thymus vulgaris L., Macronutrients, Cytokinins, Auxins, In Vitro Propagation
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.10 No.9,
September
9,
2019
ABSTRACT: The present study reports an efficient protocol for in
vitro propagation of Thymus vulgaris L., an
aromatic and medicinal plant in Morocco. Initially, we performed in vitro multiplication of Thymus vulgaris explants existing in the
laboratory and obtained from micropropagation by shoot tip culture. Afterwards,
we have evaluated the effect of six macronutrients. After that, seven
cytokinins (Kin, BAP, 2iP, DPU, Adenine, Zeatine and TDZ) in three different
concentrations (0.46, 0.93, 2.32 μM) have been evaluated to optimize cultures
multiplication and elongation. Moreover, the effect of three auxins (IAA, IBA
and NAA) at 0.57 μM, combined to 4 cytokinins (Kin, BAP, DPU and Ad.) at 0.46
μM, on shoot rooting has been studied. Thereby, MS medium has been proved the
most favorable for plantlets growing. Also, we found that the addition of
certain cytokinins, specifically 0.46 Kin, 0.46 and 0.93 BAP, 0.46 2iP, 0.46
DPU, 0.46 Ad. and 0.46 Zeat., ensures better multiplication and growth of
vitroplants. In addition, multiplication and rooting of cultures were well
optimized after addition 0.46 Kin + 0.57 IAA or NAA, 0.46 DPU + 0.57 IBA and
0.46 Ad. + 0.57 IBA combinations to the culture medium. Lastly, plantlets with roots were successfully acclimatized to
ex-vitro conditions and these latter served as a source to establish in vitro culture again.