TITLE:
Influence of Agitation Rate on the Growth of MD2 Pineapple Protocorm-Like Bodies and Shoots in Liquid-Shake Culture
AUTHORS:
Zuraida Ab Rahman, Hartinee Abbas, Ayu Nazreena Othman, Wan Zaliha Wan Sembok
KEYWORDS:
Pineapple MD2, Micropropagation, Liquid-Shake Culture, Agitation Rate
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.10 No.7,
July
31,
2019
ABSTRACT: The present study was conducted to investigate the
effect of agitation rate on the increase in fresh weight of MD2 pineapple
protocorm-like bodies (PLBs) and shoots cultured in liquid medium. PLBs were
cultured in 250 ml Erlenmeyer flasks (7 g per flask) containing MS medium and
plant growth regulators (1.5 mg/L 6-Benzylaminopurine, BAP and 0.2 mg/L
1-Naphthaleneacetic acid, NAA). The orbital shaker was set at speeds of 50, 80,
100, 120, and 150 rpm. After 40 days, the cultures shaken at 80 rpm showed the
highest fresh weight and the highest number of shoots at 76 g and 41 shoots,
respectively. A comparative study of agitation found that 80 rpm was the best
speed which enhanced both PLB and shoot formation. The findings in the present
study would be helpful in setting up large-scale in vitro mass propagation of MD2 pineapple.