TITLE:
Intestinal Handling of Glucose in Buccholzia coriacea Treated Male Wistar Rats
AUTHORS:
Olugbenga Adeola Odukanmi, Queenneth Blessing Olusegun, Samuel Babafemi Olaleye
KEYWORDS:
Buchholzia coriacea, Intestine, Glucose Uptake, Electrolytes, Rats
JOURNAL NAME:
Journal of Biosciences and Medicines,
Vol.7 No.7,
July
15,
2019
ABSTRACT: Background: Different studies have unveiled the traditional usefulness and clinical potentials of Buchholzia coriacea, a medicinal plant known for its effectiveness in lowering blood glucose. Its role in intestinal glucose uptake was investigated. Materials and methods: Thirty male Wistar rats, weighing between 100 - 120 g were used and randomly assigned into three groups of 5 rats each per experiment. Group 1: control (not treated), groups 2 and 3, were treated with 100 mg/kg (BC100) and 200 mg/kg (BC200) of Buchholzia coriacea orally respectively for 2 weeks. Fasting blood glucose, luminal and in vitro glucose levels of rats were determined by glucose oxidase method using glucometer stripes plus glucose monitoring system (Fine test glucometer(R) ). Luminal electrolytes in the in vitro study were determined by Atomic Absorption Spectrophotometry method. Data were expressed as Mean ± SEM and statistical analysis was by one way ANOVA, and p-values Results: There was no significant change in the fasting blood glucose level (mg/dl) of rats in BC100 (78.00 ± 2.16) and BC200 (76.0 ± 3.57) compared with control (79.50 ± 1.70). There was significant increase in glucose uptake (mg/dl/g tissue) in the in vivo experiments in both the ileum and jejunum of BC100 (23.08 ± 0.18; 19.68 ± 0.72) and BC200 (14.50 ± 1.02; 20.55 ± 0.45) compared with control (30.40 ± 1.01; 35.53 ± 1.45), respectively. The glucose uptake at the mucosa end of distal jejunum in the BC100 (292 ± 3.33) and BC200 (209.30 ± 2.67) decreased significantly compared with control (90.00 ± 1.50). There was a significant decrease in K+ concentration and increase in Na+ concentration at the mucosa end in the BC100 and BC200 compared with control. Conclusion: The study suggests glucose stowing in the intestinal cells in the in vivo study and inhibition of glucose transport from the in vitro study. The roles of alpha-amylase on the activity of this extract are suggested for future studies.