TITLE:
Array Comparative Genomic Hybridization as a Diagnostic Tool in Cancer
AUTHORS:
Panagiotis Apostolou, Ioannis Papasotiriou
KEYWORDS:
Cancer of Unknown Primary Origin, Array Comparative Genomic Hybridization, Cytogenetic, Cancer
JOURNAL NAME:
Journal of Cancer Therapy,
Vol.10 No.7,
July
11,
2019
ABSTRACT: The
knowledge of the primary origin of tumor is essential in designing an efficient
cancer treatment algorithm. Useful diagnostic tools enable determination of primary origin of the tumor; however the
majority of them require tissue examination.
Recent years, exploration of circulating tumor cells enabled scientists to study different parameters using the painless liquid
biopsy. The present study aimed to identify whether aCGH might be used
as a diagnostic tool in cancer detecting the primary origin of the tumor. Blood
was extracted from healthy individuals and cancer samples and CTCs isolated.
DNA extracted from the above samples and aCGH experiments followed. The samples
were blinded analyzed and then unmasked to calculate specificity and
sensitivity of the method. The sensitivity
was 94%, the specificity 88%, while the positive prediction rate of the primary
tumor was 72%. aCGH is a powerful tool in cancer diagnosis and treatment
plan with high sensitivity and specificity rates. It can be performed from
blood sample, which makes it an appropriate method for every patient, mainly for patients
with unknown origin of the primary tumor.