Article citationsMore>>

Ishihara, K., Yamaguchi, H., Hamada, H., Nakamura, K. and Nakajima, N. (2000) Asymmetric Reduction of α-Keto Esters with Thermophilic Actinomycete: Purification and Characterization of α-Keto Ester Reductase from Streptomyces thermocyaneoviolaceus IFO14271. Journal of Molecular Catalysis, B: Enzymatic, 10, 419-428.
https://doi.org/10.1016/S1381-1177(99)00114-9

has been cited by the following article:

• TITLE: Microbial Production of Chiral Hydroxy Esters and Their Analogs: Biocatalytic Reduction of Carbonyl Compounds by Actinobacteria, Agromyces and Gordonia Strains

  AUTHORS: Kohji Ishihara, Natsumi Adachi, Takumu Mishima, Chiharu Kuboki, Ayaka Shuto, Kazuya Okamoto, Manami Inoue, Hiroki Hamada, Daisuke Uesugi, Noriyoshi Masuoka, Nobuyoshi Nakajima

  KEYWORDS: Actinobacteria, Stereoselective Reduction, Carbonyl Reductase, Chiral Alcohol

  JOURNAL NAME: Advances in Enzyme Research, Vol.7 No.2, June 30, 2019

  ABSTRACT: We screened 15 Agromyces strains from the Microbacteriaceae family and 16 Gordonia strains from the Gordoniaceae family to investigate their biocatalytic ability to reduce carbonyl compounds. Two Agromyces strains (A. soli NBRC109063 and A. humatus NBRC109085) and two Gordonia strains (G. hydrophobica NBRC16057 and G. malaquae NBRC108250) grew well in 230 medium. The stereoselective reduction of various carbonyl compounds using these four strains was investigated. We discovered that these strains can reduce aliphatic and aromatic α-keto esters and an aromatic α-keto amide. On the basis of the conversion rate and stereoselectivity of the alcohols produced, G. hydrophobica NBRC16057 is a potential biocatalyst for the stereoselective reduction of α-keto esters and an aromatic α-keto amide to the corresponding chiral alcohols. Our results also suggest that the reduction of ethyl 2-methylacetoacetate by wet G. hydrophobica NBRC16057 cells in the presence of L-glutamate is useful for the production of chiral ethyl 3-hydroxy-2-methylbutanoate.