TITLE:
Rapid Quantification of Bioactive Lentinan with an Aniline Blue Fluorescent Method
AUTHORS:
Luodi Fan, Tong Li, Minghua Hu, Fangli Ma, Boyang Yu, Jiangwei Tian
KEYWORDS:
Lentinan, Triple Helical Conformation, Aniline Blue, Fluorescence Detection, Health Tonic Solution
JOURNAL NAME:
Pharmacology & Pharmacy,
Vol.10 No.6,
June
28,
2019
ABSTRACT: Lentinan is a clinically approved immune
modulator and its anticancer and immunomodulatory bioactivity is found to be
dependent on its triple helical conformation. Therefore, the development of
rapid and convenient method for determination of bioactive lentinan with triple
helical conformation holds great promise for the quality control of lentinan
healthy products. In this work, an aniline blue fluorescent method was
optimized and established to accurately and rapidly detect bioactive lentinan.
In the presence of lentinan, the fluorescence intensity of aniline blue with
404 nm excitation and 492 nm emission dramatically enhanced within 15 min in pH 10 glycine-NaOH buffer solution, which
allowed the analysis of lentinan in a very simple and fast manner. The method
allowed for the sensitive determination of lentinan in the range of 1 to 60
μg/mL with a detection limit of 0.25 μg/mL. Notably, the protocol exhibited
excellent selectivity for the determination of triple helical lentinan over
other saccharides. The method was successfully applied to the detection
of bioactive lentinan in health tonic solution, which demonstrated the method
had great potential for quality control of lentinan contained products.