TITLE:
Botanical Aspects, Caffeine Content and Antioxidant Activity of Coffea arabica
AUTHORS:
Andreia Marques dos Santos, Luis Carlos Marques, Carolina Passarelli Gonçalves, Maria Cristina Marcucci
KEYWORDS:
Coffea arabica, Botanical Characterization, Caffeine
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.10 No.6,
June
21,
2019
ABSTRACT: Brazil is the largest coffee exporter in the world.
In addition, it occupies the second position, among the consuming countries of
the drink. To investigate the chemical composition and quality of the coffee
drink from Coffea arabica species,
samples grown in the city of Ourinhos, the third most productive region in the
state of Sao Paulo, a study of its properties and characteristics was
conducted. The pharmacobotanical characteristics were investigated performed
according to usual techniques in these researches through macroscopic and
microscopic studies through cross-sections. The oil obtained for analysis was
extracted by soxhlet, and the caffeine content was measured for green grains
using High Performance Liquid Chromatography (HPLC) using as stationary phase,
column C18 and gradient mobile phase formed by 80% water and 20%
methanol. The concentration obtained was 44.983 ± 0.86 μg/mL. The antioxidant activity was measured in triplicate through the DPPH test of the in natura coffee oil, and presented antioxidant action of EC50 25.89 ± 1.16 μg/mL.