TITLE:
Can Ginger (Zingiber officinale) Aqueous Crude Extract Induce Apoptotic Pathways in Drug-Resistance Acute Myeloid Leukemia: In Vitro Study?
AUTHORS:
Nouf Saeed Al-Abbas
KEYWORDS:
Acute Myeloid Leukemia, Drug Resistance, Ginger
JOURNAL NAME:
Advances in Biological Chemistry,
Vol.9 No.3,
June
18,
2019
ABSTRACT: While chemotherapy remains to be one of the main using approaches in the
clinical treatment of acute myeloid leukemia (AML), multidrug resistance (MDR)
is considered the major obstacle that limits the therapeutic efficacy. Nowadays,
Herbal therapy as an adjuvant therapy has been used for many health problems. Ginger (Zingiber officinale) is considering one of the promising herbal spices showing high therapeutic and preventive
effects against many disorders specially cancer. In the current work, we focused on the role of ginger crude extract in fighting drug resistant AML. MTT
assay showed a significant decrease in cell viability and clear cytotoxic
effect on HL60/ADR and HL60 cell under the high concentrations (100 and 1000 μg/Ml) of
ginger extract. The flow cytometry results showed a significant apoptotic cell
death by ginger in HL60 and ADR/Hl60 and also confirmed by immunostaining of nucleus by
DAPI which showed apoptotic nuclei. Our data clearly
declared that the high concentration of ginger extract is
promising anticancer drug by induction of apoptotic cell death in HL60/ADR
cells especially in drug resistant AML.