TITLE:
DNA Finger Printing of Prosopis cineraria and Prosopis juliflora Using ISSR and RAPD Techniques
AUTHORS:
Khaled Elmeer, Ameena Almalki
KEYWORDS:
Ghaf, Ghawiaf, Polymorphism, Dendrogram and Genetic Diversity
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.2 No.4,
October
31,
2011
ABSTRACT: Diversity within and among the populations of Prosopis cineraria and Prosopis juliflora collected from different loca-tion of Qatar were explored using Inter Simple Sequence Repeat (ISSR) and Random Amplified Polymorphic DNA (RAPD) markers. A total of one hundred and nine bands were generated from twenty nine ISSR and nineteen bands from seven RAPD primers with an average polymorphism of more than ninety nine percent across all the genotypes. ISSR techniques were capable of distinguishing between P. cineraria and P. juliflora, through twenty one bands. How-ever, of the seven RAPD markers, only three bands were able to distinguish between Prosopis species. The dendro-grams for the analysis of genetic similarity show that the individuals from both species can be separated in two highly related groups. Our observations suggest that genetic variations among different accessions of Prosopis are identified using ISSR and RAPD analysis.