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H. Yamaji, T. Fukuda, J. Yokoyama, J. -H. Pak, C. -Z. Zhou, C. -S. Yang, K. Kondo, T. Morota, S. Yakeda, H. Sasaki and M. Maki, “Reticulate Evolution and Phy-logeograhy in Asarum sect. Asiasarum (Aristolochiaceae) Documented in Internal Transcribed Spacer Sequences (ITS) of Nuclear Ribosomal DNA,” Molecular Phyloge-netics and Evolution, Vol. 44, No. 2, 2007, pp. 863-884. doi:10.1016/j.ympev.2007.01.011

has been cited by the following article:

• TITLE: Interspecific Hybridization between Arisaema sikokianum and A. serratum (Araceae) Confirmed through Nuclear and Chloroplast DNA Comparisons

  AUTHORS: Hiroshi Hayakawa, Hidenori Hamachi, Kanako Matsuyama, Yuko Muramatsu, Yukio Minamiya, Katsura Ito, Jun Yokoyama, Tatsuya Fukuda

  KEYWORDS: Araceae, Arisaema, A. serratum, A. sikokianum, Chloroplast DNA, Interspecific Hybrid, Molecular Analysis, Nuclear DNA

  JOURNAL NAME: American Journal of Plant Sciences, Vol.2 No.4, October 31, 2011

  ABSTRACT: A morphologically intermediate plant between Arisaema sikokianum Franch. et Sav. and A. serratum (Thunb.) Schott has been newly found in Kochi Prefecture, Shikoku, Japan. The putative hybrid has the intermediate morphological characteristics of the parental species. Molecular analysis using PCR-RFLP of internal transcribed spacer (ITS) in nuclear DNA (nrDNA) indicates that the putative hybrid has a combined pattern of the two putative parent species. Moreover, the sequence result of chloroplast DNA (cpDNA) of the putative hybrid was identical to that of A. sikokianum. These results suggest that the putative hybrid is a hybrid between A. sikokianum and A. serratum and that it was formed by interactive gene exchanging via pollens from A. serratum to A. sikokianum. It is the first record of a hybrid between A. sikokianum and A. serratum.