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Antonio de Souza Junior, J.D., Ramos Coelho, R., Tristan Lourenco, I., da Rocha Fragoso, R., Barboso Viana, A.A., Pepino de Macedo, L.L., Mattar da Silva, M.C., Gomes Carneiro, R.M., Engler, G., Engler, J. and Grossi-de-Sa, M.F. (2013) Knocking down Meloidogyne incognita Proteases by Plant-Delivered dsRNA Has Negative Pleiotropic Effect on Nematode Vigor. PLoS ONE, 8, e85364.
https://doi.org/10.1371/journal.pone.0085364

has been cited by the following article:

• TITLE: Mitigating Root Knot Nematode Propagation on Transgenic Tobacco via in Planta Hairpin RNA Expression of Meloidogyne incognita—Specific PolA1 Sequence

  AUTHORS: Peter Nkachukwu Chukwurah, Samuel Aduse Poku, Akira Yokoyama, Ai Takeda, Masahiro Shishido, Ikuo Nakamura

  KEYWORDS: M. incognita, Nematode Resistance, N. tabacum, RNA Silencing, PolA1 Gene

  JOURNAL NAME: American Journal of Plant Sciences, Vol.10 No.5, May 30, 2019

  ABSTRACT: Root knot nematodes are top priority nematode pests that significantly constrain agricultural productivity globally especially in developing countries. However, expressing double stranded RNA (dsRNA) of essential nematode genes in susceptible plants is known to confer protection against these pests via RNA silencing. This molecular-based strategy is called host induced gene silencing (HIGS) and the selection of appropriate target nematode gene is critical to its success. In this study, therefore, we focused on root knot nematode PolA1, an essential single copy nuclear gene encoding the largest subunit of RNA polymerase I enzyme and evaluated its effectiveness as a target in conferring nematode resistance on Agrobacterium-mediated transformed tobacco plants. Transgenic tobacco expressing Meloidogyne incognita-specific (MiS) dsRNA of PolA1 gene showed significant reduction in nematode fecundity and multiplication compared to wild type plants in both T0 and T1 generations. T0 plants showed varying degrees of agronomic vigorover WT plants possibly due to varying levels of processed siRNA. However, production of MiS siRNAs in the transgenic plants coupled with significant reduction of PolA1 transcript expression in nematodes feeding on roots of transgenic plants provided evidence of HIGS. Taken together, our results show that PolA1 is a potentially effective target for HIGS-mediated reduction of root knot nematode damage on transgenic tobacco. Given the homology of our target sequence among Meloidogyne species, this protection could be broad range against other root knot nematodes aside M. incognita.