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Vincent, C., Pickering, S.J. and Johnson, M.H. (1990) The Hardening Effect of Dimethylsulphoxide on the Mouse Zona Pellucida Requires the Presence of an Oocyte and Is Associated with a Reduction in the Number of Cortical Granules Present. Journal of Reproduction and Fertility, 89, 253-259.
https://doi.org/10.1530/jrf.0.0890253

has been cited by the following article:

• TITLE: Effect of Cumulus Cells on the Efficiency of Vitrified-Thawed Immature Cattle Oocytes

  AUTHORS: Samia Mohamed Abd-El Rheem, Suzan Elsharkawy

  KEYWORDS: Cumulus Cells, Vitrified-Thawed Immature Oocytes, Cattle

  JOURNAL NAME: Open Journal of Obstetrics and Gynecology, Vol.9 No.5, May 21, 2019

  ABSTRACT: The present work was designed to examine the effect of the presence or absence of cumulus cells on the efficiency of vitrification of immature cattle oocytes. In our experiment, we had two groups: group 1, immature cattle oocytes with cumulus cells and group 2, immature cattle oocytes without cumulus cells. The two groups underwent vitrification using 20% ethylene glycol and 20% DMSO, and then thawed, and in vitro matured in TCM-199 medium and examined after 22 hours for assessment of nuclear maturation. Higher survival rate (p in-vitro maturation, the rate of MII oocytes was significantly higher (p , the cumulus cells are very important in increasing the survivability and developmental rate of vitrified-thawed immature cattle oocytes.