TITLE:
A Cross-Sectional Study on Assessment of Oxidative Stress in Coronary Heart Disease Patients in Bangladesh
AUTHORS:
Mohammad Kamruzzaman, Talita Zahin Choudhury, Tania Rahman, Laila N. Islam
KEYWORDS:
Coronary Heart Disease, Oxidative Stress, TBARS, Protein Carbonyls, Nitric Oxide
JOURNAL NAME:
World Journal of Cardiovascular Diseases,
Vol.9 No.5,
May
16,
2019
ABSTRACT: Oxidative stress is considered a central factor in endothelial dysfunction and plaque formation in coronary heart disease (CHD) patients. In this cross-sectional study, the stress-induced protein and lipid oxidation and production of signaling molecule, nitric oxide has been investigated in CHD patients in Bangladesh. A total of 140 participants were enrolled of which 70 were CHD patients, and the remaining 70 were control subjects having no cardiovascular disease. The diagnosis of CHD was made by expert physicians through characteristic electrocardiogram and troponin changes. The protein carbonyls and thiobarbituric acid reactive substances (TBARS) levels in plasma of both groups were measured by spectrophotometric methods. The plasma nitric oxide levels were measured by reaction with Griess reagent. Statistical non-parametric tests and t-tests were used to analyze the results between the CHD patients and controls. In the patients, the mean protein carbonyls and TBARS, two important markers of oxidative stress, were 2.87 ± 0.97 nmol/mg and 6.92 ± 3.56 nmol/mL, respectively, which were significantly higher than in the control subjects, 1.71 ± 0.82 nmol/mg and 3.15 ± 1.42 nmol/mL, respectively. Assessment of the cardiovascular signaling molecule nitric oxide showed significantly lower value in the CHD patients (6.29 ± 4.78 μM) compared to the controls (10.75 ± 6.03 μM). There was a significant negative correlation between plasma TBARS and total plasma protein, and a weak negative correlation between TBARS and nitric oxide levels in the CHD patients. Evaluation of the smoking habit strongly suggested that smoking was a risk factor for the development of CHD among the Bangladeshi population. These results provide an overview of oxidativestress-mediated damages on proteins, lipids and effects on signaling molecule nitric oxide in CHD patients.