TITLE:
Study on Optimizing Nutrition and Fermentation Conditions for Compound Bacillus spp.
AUTHORS:
Yanyan Li, Yuxiang Xu, Wenhao Li, Yong Yang, Lin Wang, Jun Yu, Changjun Wang, Xihong Li
KEYWORDS:
Compound Bacillus spp., Spore Yield, Medium Optimization, Fermentation Optimization
JOURNAL NAME:
American Journal of Molecular Biology,
Vol.9 No.2,
April
28,
2019
ABSTRACT: In order to improve the spore yield of compound Bacillus spp. (B. amyloliquefaciens, B.
laterosporus and B. megaterium),
the effects of nutrient conditions including carbon source, nitrogen source,
mineral salt and fermentation conditions including the inoculum age,
inoculation amount, loading volume of liquid and initial pH on the spore yield
were studied. The results indicated that the optimized medium was glucoses 20
g/L, soybean meal 30.0 g/L, K2HPO4 1.0 g/L; fermentation
temperature is 37℃, the inoculum age 12 h,
initial pH 7.0, 2% inoculation amount, loading volume of liquid 20 mL/250 mL.
Under the optimized conditions of culture medium and fermentation for compound Bacillus spp., spore yield was 10.24 times more than the initial medium, and the spore
formation rate reached more than 90%.