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Col, E., Caron, C., Chable-Bessia, C., Legube, G., Gazzeri, S., Komatsu, Y., Yoshida, M., Benkirane, M., Trouche, D. and Khochbin, S. (2005) HIV-1 Tat Targets Tip60 to Impair the Apoptotic Cell Response to Genotoxic Stresses. The EMBO Journal, 24, 2634-2645. https://doi.org/10.1038/sj.emboj.7600734

has been cited by the following article:

• TITLE: Tip60 Tumor Suppressor Requires Its NLS Motif to Interact with Importin α

  AUTHORS: Eun Jeoung Lee, Sung Hwa Shin, Sang Sun Kang

  KEYWORDS: Tip60, Importin α, Nuclear Localization Sequence, Protein-Protein Interaction, HAT Activity, Cell Survival

  JOURNAL NAME: CellBio, Vol.8 No.1, March 30, 2019

  ABSTRACT: Tip60 is a specific member of MYST (Moz-Ybf2/Sas3-Sas2-Tip60) family of nuclear histone acetyltransferases (HAT). It is essential for cellular survival, differentiation, and metabolism. A putative canonical NLS motif between the chromo domain and the zinc finger of Tip60 was identified. Here we show evidence that Tip60 is associated with importin α as its substrate and transported from cytoplasm to the nucleus. Pull down assay revealed that Tip60 was physically associated with importin α both in vivo and in vitro. Confocal microscopic observation showed that Tip60 and importin α were co-localized with each other. The localization of Tip60 to the nuclear and its interaction with importin α was disrupted when its putative NLS motif for binding to importin α was mutated (219RKRK222 → 219AAAA222). However, attachment of this putative NLS motif to a cytoplasmic protein (YAP 1-210 fragment) promoted its nuclear localization. Based on transient transfection, Tip60 NLS motif mutant showed a substantial reduction in self-acetylation, HAT activity, and apoptotic ability whereas wild type Tip60 did not show such reduction. Taken together, our results demonstrate that importin α transports Tip60 from the cytoplasm to the nucleus through binding to the putative NLS motif of Tip60 for its tumor suppressing function.