TITLE:
Modification of Periodate Oxidation Method to Produce HRP-IgG Conjugate and Test its Stability Overtime
AUTHORS:
Nataliia Pavliuchenko, Victoria Hazarnian, Marcel Bassil
KEYWORDS:
Antibody, Conjugation, Horseradish Peroxidase (HRP), Indirect ELISA, Periodate-Mediated Glycoprotein Oxidation
JOURNAL NAME:
American Journal of Molecular Biology,
Vol.9 No.2,
April
16,
2019
ABSTRACT: Goat anti-Rabbit Horseradish Peroxidase (HRP) secondary conjugate was produced using a modified periodate oxidation method. The obtained conjugate was tested in the quality control techniques of therapeutic proteins. To determine the working dilution, titration of the prepared conjugate was performed in Indirect Enzyme-Linked Immunosorbent Assay (ELISA) and found to be 1:5000. This dilution was further tested in Western Blot analysis. The secondary conjugate was kept at 4°C for one month and its stability was verified by Western Blot and Indirect ELISA techniques.