TITLE:
Synergistic Effects of Targeting Survivin and CDK1 on Nasopharyngeal Carcinoma in Vitro and in Vivo
AUTHORS:
Hongtao Zhou, Xinping Chen, Shuping Chen, Junhong Cai, Jiye Zhang, Shengmiao Fu
KEYWORDS:
Surviving, Cyclin-Dependent Kinase1, RNA Interference, CNE-2
JOURNAL NAME:
Journal of Cancer Therapy,
Vol.10 No.4,
April
9,
2019
ABSTRACT: Background: To explore the impact of pU6-based tandem survivin
and CDK1-specific short hairpin RNA on the biological behaviors of CNE-2 nasopharyngeal
carcinoma cells in vitro and in vivo. Patients and Methods: The
vectors of pU6-survivinshRNA, pU6-CDK1shRNA and
pU6-survivinshRNA-CDK1shRNA were constructed and
transfected into CNE-2 cells with Lipofectamine TM 2000, respectively. The
mRNAs and proteins of CDK1 and survivin were determined by RT-PCR and Western
blotting, accordingly. MTT assay was employed to evaluate the proliferation of
CNE-2 cells, and flow cytometry was performed to determine the apoptosis of CNE-2 cells.
The effects of interfering survivin and CDK1 on tumorigenesis were evaluated by
tumor xenografts experiments. Results: Effective plasmids were
successfully constructed knocking down survivin and/or CDK1. The proliferation
inhibition of CNE-2 cells by pU6-survivinshRNA-CDK1shRNA (32.5%)
was higher than that of by pU6-survivinshRNA (25.6%) and pU6-CDK1shRNA (15.6%), and apoptosis in CNE-2
cells simultaneously interfering survivin and CDK1 (15.2%) dramatically
increased when compared to those of interfering survivin (5.4%) or CDK1 (4.7%)
alone. Furthermore, simultaneously interfering survivin and CDK1 is more
effective than interfering alone component in inhibiting tumor
growth of fBalb/C nude mice xenografted with CNE-2 cells. Conclusion: The results altogether indicate that interfering survivin and
CDK1simutaneously can produce synergistic effects of anti-nasopharyngeal carcinoma,
which could be a potential therapeutic method.