TITLE:
Bacterial Surface Layer Proteins: A Promising Nano-Technological Tool for Bio-Sensing Applications
AUTHORS:
Anjali Sharma, Barkha Singhal
KEYWORDS:
Surface Layer Proteins, Nano-Patterning, Self-Assembly, Liposome, Biosensors
JOURNAL NAME:
Advances in Bioscience and Biotechnology,
Vol.10 No.3,
March
26,
2019
ABSTRACT: The phenomenal rise in the demand of biosensors
accelerated their rapid development and immersive applications in the myriads
of fields. The essential requirement of developing efficient bio-sensing
platform is to find stable well organized interfacial architecture that can serve as an excellent matrix
for binding and recognizing biomolecules. In this context, the enormous
potential has been envisaged in surface layer proteins that represented
themselves as most primitive and simplest self-assembled system with repetitive
physicochemical properties for the molecular functionalization of surfaces and
various interfaces. The prominence of S-layer proteins has been broadened by
integrating genetic engineering approaches for the fine tuning of functional
groups and protein domains in geometrically
well-defined manner. The efficient and stable binding of various nanomaterials
with S-layers in regular arrays has led to paradigmatic shift in their
nano-biotechnological sensing applications. More recently, functional S-layer
supported lipid membranes have been generated through covalent binding of lipid
molecules either with native or recombinant S-layer proteins at nano-scale
dimensions serving as “proof of concept” for the development of bio-sensing platform. Thus,
in the light of benefits conferred by surface layer proteins for the
development of highly efficient biosensors, an exciting path has been opened
for broadening their translational applications in drug delivery, disease
diagnosis, vaccines development, lab-on-chip devices etc. Therefore, this review intends to describe about the
importance of surface layer proteins in the development of biosensors.