TITLE:
Study on the Multi-Component Quantitation of Cortex Moutan and Its Intestinal Absorption Characteristics
AUTHORS:
Mei Ge, Xihong Hou, Shimeng Li, Kunpeng Liu, Ziyi Han, Siyu Hao, Zhongliang Zeng, Yingchun Zhang
KEYWORDS:
Cortex Moutan, Paeonol, Paeoniflorin, Everted Intestinal Sac, HPLC-MS
JOURNAL NAME:
Pharmacology & Pharmacy,
Vol.10 No.3,
March
25,
2019
ABSTRACT: Cortex Moutan (Paeonia suffruticosa Andr.) is a common
traditional Chinese medicine and has been widely used in clinic for 2000 years
in China. As sources for this crude drug are always mixed with other species, many cultivars on herbal market may lead to quality instability. Multi-component
quantitative analysis is an efficient method to reflect chemical profiles of
herb medicine and is always taken as the main method
for quality evaluation. So, the aim of this work
is to develop analytical method to quantify paeonol, paeoniflorin, gallic acid,
oxypaeoniflorin, benzoylpaeoniflorin and paeonolide in Cortex Moutan (CM) to
evaluate the chemical qualities of CM from different species or cultivars. Besides,
we also study the intestinal absorption characteristics of paeonol and paeoniflorin
for further pharmacological evaluation. In the present study, all of the
standard markers were performed on an Ecosil C18 (250 mm × 4.6 mm, 5
μM, Lubex Co., Guangzhou, China) with linear gradient elution of 0.2% formic
acid water and acetonitrile. The proposed method was applied to analyze 50
batches of samples with acceptable linearity (R2, 0.9995 - 0.9999), precisions (RSD, 0.47% - 2.08%), repeatability (RSD, 039% - 2.63%), stability (RSD, 0.52% - 2.45%), and recovery (RSD, 0.72% - 3.03%) of the six compounds. Furthermore, the Hierarchical Cluster
Analysis was applied to classify the 50 samples based on contents of the six
compound markers. The results obtained from multi-component quantification of
CM clearly indicated that CM originated from P. suffruticosa and P. ostii presented different chemical properties, and that samples from the two materials could be gathered into one branch,
respectively, while CM sourced from cultivars of P.
suffruticosa showed great variety on chemical quality. The results from Hierarchical
Cluster Analysis implied that the established method could be used as a
powerful tool for the quality evaluation of CM. The intestinal absorption study
indicated that the intestinal absorption activities for paeoniflorin and
paeonol showed an increasing absorption with time. Paeonol had lower absorption
rate (6.69% - 15.93%) than that of paeoniflorin (19.0% - 30.70%). As a result, the established method is suitable for the quality evaluation
of CM. The results of intestinal absorption characteristics of paeonol and paeoniflorin
offer an insight for pharmacological evaluation and clinical efficacy research
of CM.