TITLE:
Investigation of the Effects of Zebularine on Caspase-3 and Caspase-9 Involved in Anticancer and Apoptotic Mechanisms in SKBR3 Breast Cancer Cell Line
AUTHORS:
Onur Eroglu, Merve Celen
KEYWORDS:
SKBR3, Zebularine, Apoptosis, Breast Cancer
JOURNAL NAME:
Journal of Cancer Therapy,
Vol.10 No.3,
March
12,
2019
ABSTRACT: The aim of this study is to investigate the anti-proliferative effects of
Zebularine on caspase-3 and 9 genes by methylation and protein expression on
SKBR3 cells. The SKBR3 cells were treated with Zebularine at different concentrations
(0 - 140 μM) for 24 - 96 hours and the effects on the cell viability were
shown. The effects on the cell migration and cell transformation were also
demonstrated at the specified dose (IC50 = 40 μM). At the same time, the HRM
method and western-blot analysis were used to understand the effects in the
apoptotic mechanism. According to the obtained results, it was observed that
Zebularine significantly decreased the cell proliferation, cell migration and
the cell growth (p it was
observed that Zebularine caused a decrease in both caspase-3 and 9 methylation rates at the 72nd hour. However, both reductions
in the methylation levels didn’t cause a significant change (p > 0.05). At
the same time, it was detected by the western-blot analyses that there was a
time dependent increase in caspase-3 genes while a decrease was detected in the
caspase-9 gene in progress of time. The obtained results showed that the
methylation changes occurring in the caspase genes weren’t related to the
protein levels. According to these results, supportive results are
obtained showing that the Zebularine can be
used in chemotherapy and that this study is unique
since it is the first study in the literature
intending to investigate the effects of Zebularine on the SKBR3 cells.