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Choudhary, G.S., Al-Harbi, S. and Almasan, A. (2015) Caspase-3 Activation Is a Critical Determinant of Genotoxic Stress-Induced Apoptosis. Methods in Molecular Biology, 1219, 1-9. https://doi.org/10.1007/978-1-4939-1661-0_1

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• TITLE: Anti-Tumor Effect and Mechanism of Parthenolide in Gastric Cancer Cellline BGC-823

  AUTHORS: Wenhao Zhu, Hanni Zhang, Yunliang Guo, Keli Ge, Yonghong Zhou

  KEYWORDS: Parthenolide, Gastric Cancer, Cell Proliferation, Cell Cycle, Apoptosis

  JOURNAL NAME: Open Access Library Journal, Vol.6 No.3, March 8, 2019

  ABSTRACT: Objective: To study the anti-tumor effect and mechanism of parthenolide in gastric cancer cell BGC-823. Methods: The cck8 assay was used to detect the changes of BGC-823 cells viability after treatment with different con-centrations of parthenolide at different time points. The proliferation ability of BGC-823 cells was detected by clone formation assay. And the cell cycle and apoptosis were measured by flow cytometry. Meanwhile, to detect the different in intracellular ROS production levels, the fluorescence assay was used. And with the help of western blotting, cell cycle- and apoptosis-related protein expression can be detected. Results: Parthenolide could inhibit the viability of BGC-823 cells in a dose- and time-dependent manner (P