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Jovel, J., Patterson, J., Wang, W., Hotte, N., O’Keefe, S., Mitchel, T., Perry, T., Kao, D., Mason, A.L., Madsen, K.L. and Wong, G.K. (2016) Characterization of the Gut Microbiome Using 16S or Shotgun Metagenomics. Frontiers in Microbiology, 7, 459.
https://doi.org/10.3389/fmicb.2016.00459

has been cited by the following article:

• TITLE: Comparison of in Clinic-Based Fecal Microbiome Collection Techniques for Increase in Study Participation and Utilization of Microbiome Analysis

  AUTHORS: Benjamin D. Besasie, Dimpy P. Shah, Robin J. Leach, Michael A. Liss

  KEYWORDS: Fecal Microbiome, Stool, Collection Technique, Swab, Glove, Urology, Digital Rectal Exam, 16S rRNA Gene Sequencing

  JOURNAL NAME: Open Journal of Urology, Vol.9 No.3, March 8, 2019

  ABSTRACT: We compared the collection techniques of fecal specimens for DNA extraction and fecal microbiome analysis by utilizing the glove from a standard-of-care digital rectal exam (DRE) and the rectal swab from a pre-prostate biopsy bacterial rectal culture collected in clinical care settings. DNA yield from the swab technique compared to the glove technique yielded similar amounts of DNA (18.1 vs. 13.1 ng/μL, p = 0.06), slightly favoring the swab technique. However, utilizing DNA yield cutoffs of 15 ng/μL (37% vs. 29%, p = 0.18) and 30 ng/μL (15% and 9%, p = 0.16), we identified no differences in yield between the swab versus glove technique, respectively. Absorbance values for overall DNA quality were significantly different in favor of the glove technique (mean 1.6 vs. 2.0, p