TITLE:
Comparison of in Clinic-Based Fecal Microbiome Collection Techniques for Increase in Study Participation and Utilization of Microbiome Analysis
AUTHORS:
Benjamin D. Besasie, Dimpy P. Shah, Robin J. Leach, Michael A. Liss
KEYWORDS:
Fecal Microbiome, Stool, Collection Technique, Swab, Glove, Urology, Digital Rectal Exam, 16S rRNA Gene Sequencing
JOURNAL NAME:
Open Journal of Urology,
Vol.9 No.3,
March
8,
2019
ABSTRACT: We compared the collection techniques of fecal specimens for DNA extraction
and fecal microbiome analysis by utilizing the glove from a standard-of-care digital rectal exam (DRE) and the rectal swab from a
pre-prostate biopsy bacterial rectal culture collected in clinical care settings.
DNA yield from the swab technique compared to the glove technique yielded
similar amounts of DNA (18.1 vs. 13.1 ng/μL, p = 0.06), slightly favoring the
swab technique. However, utilizing DNA yield cutoffs of 15 ng/μL (37% vs.
29%, p = 0.18) and 30 ng/μL (15% and 9%, p = 0.16), we identified no differences
in yield between the swab versus glove technique, respectively. Absorbance
values for overall DNA quality were significantly different in favor of
the glove technique (mean 1.6 vs. 2.0, p