TITLE:
Effect of IRAK1 on Apoptosis and Necroptosis of Hepatoma Cell Line SK-Hep1
AUTHORS:
Zhijia Pan, Xiaolei Zhang, Changshi Qian, Xiaochen Liu, Xinglin Jin
KEYWORDS:
IRAK1, Apoptosis, Necroptosis, FADD, RIP1, TRAF6
JOURNAL NAME:
Chinese Medicine,
Vol.10 No.1,
February
13,
2019
ABSTRACT: Interleukin I receptor associated kinase 1 (IRAK1)
is a downstream signal molecule of activated MyD88 recruitment, which can
activate Fas associated death domain protein (FADD) to induce apoptosis. IRAK1
can also activate tumor necrosis factor-related factor 6 (TRAF6) and induce the
expression of a series of downstream specific genes. IRAK1 is an essential
factor in the induction of mitochondrial division and necroptosis. In the
current study, RNAi technique was used to silence IRAK1, and the apoptosis and necroptosis
rate of SK-Hep1 cells were detected by flow cytometry. The apoptosis and the
necroptosis pathway of hepatoma SK-Hep1 cells were blocked separately, and the
expressions of FADD, RIP1 and TRAF6 genes were silenced separately. The results showed when the expression of
IRAK1 was down-regulated, the apoptosis and necroptosis rate of SK-Hep1
cells were significantly increased. With silenced FADD, RIP1 and TRAF6,
respectively, the expression of IRAK1 protein had no significant change.
However, the expression of IRAK1 mRNA decreased significantly (p