TITLE:
Bone Marrow Cells Obtained from Old Animals Differ from the Young Animals Cells in Their Ability to Divide and in Response to the Presence of Liver Fibrosis in Primary Culture
AUTHORS:
S. L. Ohiienko, A. I. Bozhkov, A. Yu. Bondar, E. G. Ivanov, I. A. Ionov
KEYWORDS:
Bone Marrow, Aging, Proliferation, Lymphocyte, Intracellular Ca2+
JOURNAL NAME:
Advances in Aging Research,
Vol.8 No.1,
January
31,
2019
ABSTRACT: Dynamics
of bone marrow cells number (BMC) in the primary culture isolated from young (3
months) and old (20 months) Wistar rats was investigated. Proliferative activity of BMC of old animals was
2 times higher than that of young animals in the primary culture. Such
superiority of the proliferative activity of BMC in the primary culture
obtained from old animals is associated with the ability to actively divide
lymphocytes and longer “lifespan” of segmented neutrophils obtained
from old animals. It should be noted, that the lymphocytes of young animals did
not proliferate in the primary culture. The content of intracellular calcium in
BMC in the cells of old animals was 3 times higher compared with cells of young
animals, which revealed the relationship of intracellular calcium and
proliferative activity of BMC. Induction of liver fibrosis led to an increase
in the lymphocyte content in young animals by 167%, and in old ones only by
26%, while the lymphocytes of young animals acquired the ability to proliferate
in the primary culture. It has been suggested that such differences in the
behavior of BMC in primary culture obtained in young and old animals reflect
differences in the BMC microenvironment of young and old animals, which leads
to changes in the epigenetic-metabolic characteristics of BMC.