TITLE:
Tracing of Antibiotic-Resistant Bacteria Isolated from Semen of Iraqi Males with Primary Infertility
AUTHORS:
Mohemid M. Al-Jebouri, Salih A. Mdish
KEYWORDS:
Antibiotic Resistance, Bacteriospermia, Bacteria, Iraq
JOURNAL NAME:
Open Journal of Urology,
Vol.9 No.1,
January
17,
2019
ABSTRACT: Objectives: The main objective of this study was to investigate the bacterial
infections of seminal fluid in males with primary infertility. The patients were
referred to and/or visiting the Urology Department in Salah Al-Deen Teaching
Hospital. Methods: The present study was carried out from May 2017 to
June 2018 and the number of the patients group was 60. The patients aged
between 20 to 50 years. Semen was collected from infertile men of a couple
that female failed to become pregnant after one year of regular and unprotected
intercourse of marriage and submitted for seminal fluid analysis for the
bacteriological analyses. The control group consisted of 50 fertile male who
were selected randomly from Tikrit City during the period of this study. Semen
culture was done for all the patients and the control group. Results: There were 30 patients who suffered from bacteriospermia and 16 of them
(53.3%) were infected with gram positive organisms, while only 14 (46.7%)
patients were infected with gram negative organisms. The most common organism
was Staphylococcus aureus which was isolated from bacteriospermia. Escherichia coli, Enterococcus faecalis, Klebsiella pneumoniae, S. saprophyticus, Proteus mirabilis and Niesseria gonorrhoae were also isolated and the
frequencies of isolation were 23.3% (7), 13.3% (4), 10.0% (3), 10.0% (3), 6.6%
(2), and 6.6% (2) respectively. The present study showed many antibiotics
were not active against gram positive bacteria isolated e.g. the resistance frequenciy
to rifampcin, vancomycin and clindamycin was 48%, 44% and 40%
respectively. Conclusions: The gram positive organisms were more prevalent
than gram negative organisms in patients with bacteriospermia and the isolationfrequencies
of two groups were 16 (53.3%) and 14 (46.7%) respectively.
The most effective antimicrobial agents were amikacin, ciprofloxacin and levofloxacin
and the least effective one was ceftazidime, whereas moxifloxacin, norfloxacin, gentamicin, azithromycin, moxifloxacin, norfloxacin, cefotaxime,
and ceftriaxone were effective at different levels.