TITLE:
Obtaining the Fluorescent Chitosan for Investigations in the Analytical Ultracentrifuge
AUTHORS:
Murodkhon R. Kodirkhonov
KEYWORDS:
Polysaccharide, Chitosan, Analytical Ultracentrifuge, Fluorescein-5-Isothiocyanat, Fluorescent Chitosan, Labeling, Sedimentation, Degree of Substitution, UV Absorption
JOURNAL NAME:
Advances in Biological Chemistry,
Vol.9 No.1,
January
16,
2019
ABSTRACT: 1) In
order to achieve the visibility of the chitosan macromolecule for the UV
optical system of the analytical ultracentrifuge on investigation of the
molecular characteristics and polymers interactions, the labeling of chitosan
by a new fluorophore of fluorescein-5-isothiocyanat was carried out. 2) Samples
of fluorescent chitosan with two different degrees of fluorophore substitution
and various degrees of acetylation were obtained. 3) The labeled chitosans with
the fluorescein-5-isothiocyanat allowed estimating the sedimentation
coefficient and the molecular characteristic in the analytical ultracentrifuge.
4) The sensitivity of the UV-optical system of the analytical ultracentrifuge
for the obtained fluorescent samples of chitosan relatively to the fixation of
the meniscus and the influence of the wavelength and rotation speed were estimated.