TITLE:
Molecular Characterization of Some Amylolytic Srains of Bacteria Isolated from Peels and Soils from Two Cassava Dumpsites
AUTHORS:
A. D. Victor Ayansina, A. Olasoji Adelaja, John Baba
KEYWORDS:
Cassava, PCR, Amylase, Hydrolysis, Bacillus
JOURNAL NAME:
Open Journal of Applied Sciences,
Vol.9 No.1,
January
14,
2019
ABSTRACT: Samples
of peels and soils from cassava waste sites were collected from cassava
processing factory in Oyo town, Oyo State and also a dumpsite in Alaya village,
Aiyedire local government, Osun State, Nigeria. Bacterial isolation was carried
out using the pour plate method to obtain amylolytic bacterial species capable
of hydrolyzing starch. Fourteen bacterial isolates that were most responsive to starch hydrolysis were
selected from both sampling sites for molecular investigations. DNA of the
isolates was extracted and subjected to a cocktail mix and condition for PCR
which was purified using two universal primers and afterwards the PCR product
was used for the polymorphism through electrophoresis. Bacterial isolates were
identified based on their genetic sequences and results showed Bacillus subtilis (21.44%) to be the most frequently occurring specie. There was the prevalence
of two similar strains Bacillus subtilis MML2483 and B. subtilis MML2411 which
was isolated from the different sampling sites. Some other bacterial strains
included Bacillus olivae BRB18, B. licheniformis HT-26-B1, B. cereus H17, B. safensis MS40, B. pumilus 07