TITLE:
Component Analysis of Anthocyanins in Petals at Different Flowering Stages of Three Rosa rugosa Hybrid Cultivars
AUTHORS:
Mingyuan Zhao, Xiaoming Sui, Yu Wang, Zongda Xu, Xiaoyan Yu, Xu Han
KEYWORDS:
Rosa rugosa, Flower Color, Anthocyanin, HPLC
JOURNAL NAME:
Advances in Bioscience and Biotechnology,
Vol.10 No.1,
January
8,
2019
ABSTRACT: The species and contents of anthocyanins in plant
petals can make plants appear pink, red, violet and blue, etc., and play a
major role in the coloration of plants. In this study, the species and contents
of anthocyanins in the petals of three R. rugosa hybrid cultivars, R.
rugosa “Zizhi”, R. rugosa “Fenzizhi” and R. rugosa “Baizizhi”,
were analyzed, and the direct cause of the differences in flower color of three R. rugosa hybrid cultivars was inferred. This paper provides a reference
for the coloration mechanism and flower color breeding of R. rugosa. The
specific methods are as follows: the petals of five flowering stages of three R.
rugosa hybrid cultivars were used as materials, and the types and contents
of anthocyanins contained in them were qualitatively and quantitatively
analyzed by high performance liquid chromatography (HPLC). The same six kinds
of anthocyanins were identified in R. rugosa “Zizhi” and R. rugosa “Fenzizhi”, mainly based on the
diglycoside of paeoniflorin and cyanidin. The relative contents of the two
anthocyanins were higher at budding stage and initial opening stage. In the
different flowering stages of R. rugosa “Zizhi”, the content of Pn3G5G
was up to 4280.84 ± 20.82 μg·g-1, and the content of Cy3G5G was up
to 789.41 ± 1.21 μg·g-1. In R. rugosa “Fenzizhi”, the highest
content of Pn3G5G reached 1293.50 ± 17.64 μg·g-1, and the content of
Cy3G5G was up to 358.86 ± 3.94 μg·g-1. It could be speculated that
the difference in the contents of Pn3G5G and Cy3G5G was the main reason for the
difference in coloration between the petals of R. rugosa “Zizhi” and R.
rugosa “Fenzizhi”. A total of five species of anthocyanins were identified
in R. rugosa “Baizizhi” and their contents were relatively low. Compared
with R. rugosa “Zizhi” and R. rugosa “Fenzizhi”, the presence of
Cy3G was not detected. Therefore, we speculated that the two reasons above might
be responsible for the visual white flowers of R. rugosa “Baizizhi”.