TITLE:
Screening and Application of Phthalic Acid Degrading Bacteria
AUTHORS:
Wenhao Li, Xiaoqiong Yang, Gaodong Li, Cheng Li, Yuhan Xu, Jingguo Sun, Changjun Wang, Shunyi Li, Xin Ma, Qin Wang, Shouwen Chen, Jun Yu, Yong Yang
KEYWORDS:
O-Phthalic Acid, Allelopathic Autotoxic Substance, Tobacco
JOURNAL NAME:
Open Journal of Applied Sciences,
Vol.8 No.12,
December
20,
2018
ABSTRACT: O-phthalic acid is a kind of important
pollutant, which accumulates in the environment with the extensive use of
plastics and other products. Meanwhile, phthalic acid is one of the high
content of allelopathic autotoxic substances secreted by tobacco. The
accumulation of phthalic acid in soil is an important cause of tobacco
continuous cropping effect. In order to degrade phthalic acid accumulated in
environment, the barrier effect of tobacco continuous cropping caused by
phthalic acid accumulation in soil can be removed. A strain capable of
degrading phthalic acid was isolated from sludge of sewage treatment plant and
compared with 16 s DNA. The homology between this strain and Enterobacter sp.
is 99%. The optimum growth conditions are as follows: pH7 at 30°C, 500 mg/L of
o-phthalic acid, inoculation concentration ≥ 1.2% and its highest degradation
rate of o-phthalic acid is 74%. The results of pot experiment showed that the
degradation efficiency of o-phthalic acid in soil was about 40%, which
alleviated the inhibitory effect of o-phthalic acid accumulation on tobacco
growth.