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Shigechi, H., Fujita, Y., Koh, J., Ueda, M., Fukuda, H. and Kondo, A. (2004) Energy-saving direct ethanol pro-duction from low-temperature-cooked corn starch using a cell-surface engineered yeast strain co-displaying glu-coamylase α-amylase [J]. Biochem EngJ, 18, 149-153.

has been cited by the following article:

• TITLE: Optimization of clear liquid fermentation condition for ethanol production from Canna edulis Kerl

  AUTHORS: Ying Shen, Qing-li Tang, Tian-Xiang Wu

  KEYWORDS: Alcohol; Canna edulis Kerl; Clear Liquid Fermentation; Orthogonal Design

  JOURNAL NAME: Natural Science, Vol.2 No.2, March 3, 2010

  ABSTRACT: In this paper, clear liquid fermentation condition for ethanol production from Canna edulis Kerl with orthogonal design was studied. We have studied five factors that influenced clear liquid fermentation condition for ethanol production from Canna edulis Kerl by the single-factor test. The five factors were α-amylase amount, glu-coamylase amount, pH, nitrogen and auxiliary materials amount. Selected the best amounts of α-amylase, glucoamylase, pH, nitrogen source and auxiliary materials by means of the singe- factors test. And then, we used the Orthogonal test to optimize the fermentation process. The study showed that the best fermentation condi-tions were α-amylase amount 7 u/g C.eduli, glu-coamylase amount 145u/g C. edulis, pH 4.4 and the amount of urea 0.08%, 1% of rice bran as auxiliary materials. The rate of alcohol which was produced by the best fermentation conditions is 21.5% that increased by 1.3% than the rate of alcohol by the single-factor test.