TITLE:
Isolation, Identification and Characterization of Gram Negative Bacteria from Popular Street Food (Chotpoti) at Savar Area, Dhaka, Bangladesh
AUTHORS:
A. H. Happy, M. G. Alam, S. Mahmud, M. A. S. Imran, M. H. Rony, M. A. A. Azim, M. M. Islam, M. K. D. Sarker, P. Akter, G. C. Mondol, T. Hossain, M. M. Rahman, M. M. Islam, A. Roy, S. Das, M. R. Ahmed, M. E. Uddin
KEYWORDS:
Isolation, Street Foods, Identification, Antibiotic Sensitivity and Public Health Hazards
JOURNAL NAME:
Open Access Library Journal,
Vol.5 No.11,
November
23,
2018
ABSTRACT: Chotpoti is a popular street food in the major areas of Bangladesh. Street foods play an important role in people’s daily food options as well as their regular nutritional requirements are dependent on these foods, as their ever-growing busy schedule takes away the opportunity to eat of homemade food. Due to the expedient availability, these street foods are one of the primary food options for city peoples. The present study was undertaken to investigate the microbiological quality of Chotpoti sold by different street vendor at Savar area, Dhaka, Bangladesh. Microbial quality was assessed by total viable count (TVC), total coliform count (TCC); samples were inoculated into various selective media such as Nutrient agar, Eosin Methylene Blue (EMB) agar, Salmonella Shigella (SS) agar, Thiosulfate Citrate Bile Salts Sucrose (TCBS) agar and Mac Conkey’s agar. Gram negative bacteria were isolated and identified from the samples. The TVC in Chotpoti sample was ranged from 6 × 105 CFU/g to TNTC and TCC was ranged from 6 × 105 CFU/g to 3 × 106 CFU/g. Antibiotic sensitivity test showed that the isolates were sensitive to antibiotics such as antibiotic against Streptomycin, Chloramphenicol. Data of this study indicated that Chotpoti sold at street around Savar region harbor multidrug resistant food borne bacteria which might cause public health hazards.