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Inui, M., Suda, M., Kimura, S., Yasuda, K., Suziki, H., Toda, H., Yamamoto, S., Okino, S., Suziki, N. and Yukawa, H. (2008) Expression of Clostridium acetobutylicum Butanol Synthetic Genes in Escherichia coli. Applied Microbiology and Biotechnology, 77, 1305-1316.
https://doi.org/10.1007/s00253-007-1257-5

has been cited by the following article:

• TITLE: Investigation of Biobutanol Efficiency of Chlorella sp. Cultivated in Municipal Wastewater

  AUTHORS: Melih Onay

  KEYWORDS: Microalgae, Chlorella, Biobutanol, Biofuel, Municipal Wastewater

  JOURNAL NAME: Journal of Geoscience and Environment Protection, Vol.6 No.10, October 23, 2018

  ABSTRACT: Many strains of microalgae can grow in wastewaters through their ability to utilize inorganic nitrogen and phosphorus in wastewater. The content of municipal wastewater changes from a location to others. Biofuel production from municipal wastewater has gained huge importance due to progresses in cultivation of microalgae in wastewaters. Biobutanol is produced by the acetone-butanol-ethanol (ABE) fermentation. In this study, we examined the biobutanol production efficiency of Chlorella sp. DEE006 which is cultivated in the municipal wastewater in flat-photobioreactor. Growth of microalgae was monitored at 680 nm using spectrophotometer and the biomass was also pre-treated with acidic hydrolysis (1 M H2SO4). Total carbohydrate and protein contents were measured. Fermented microalgae samples were taken for calculation of biobutanol concentration. We obtained both high biobutanol content (6.23 ± 0.19 g·L−1) and high bioethanol yield 0.16 ± 0.005 g (g sugar)−1. 50% wastewater had the highest biomass concentration (1930 ± 11 mg/L) among the wastewaters with five various concentrations. It had the highest biomass productivity with 0.28 ± 0.001 g L−1d−1. Also, it obtained the highest carbohydrate and protein concentration with 0.80 ± 0.02 gL−1 and 0.95 ± 0.01 gL−1, respectively. According to our results, Chlorella sp. DEE006 can be used for large scale biobutanol production in the future.