TITLE:
Prevalence, Identification and Antibiotic Susceptibility of Enterococcus Species Isolated from Chicken and Pigeon Meat in Gazipur Area of Bangladesh
AUTHORS:
Avijit Banik, Noor Mohammad, Tanzina Akter, Kaniz Fatema, Maruf Abony
KEYWORDS:
Enterococcus, Enterococci from Chicken and Pigeon, Antibiotic Sensitivity, Gazipur Area in Bangladesh
JOURNAL NAME:
Open Journal of Medical Microbiology,
Vol.8 No.3,
September
18,
2018
ABSTRACT: Aims: The aims of this work undertaken to investigate and identify the prevalence of Enterococcus spp isolated from Chicken and pigeon meat and determine the using antibiotics sensitivity against isolates for human safety. This study was conducted in center of excellence lab in Microbiology Department of Primeasia University, Banani, Dhaka-1213, Bangladesh during August, 2017 to March, 2018. Methodology and Results: Twenty four samples were collected in pre-sterilized zip-lock bags from different Gazipur area in different local meat vendors. All samples were transported to the Microbiology Laboratory of Primeasia University and analyzed according to standard analysis methods. Total viable count (TVC) and Total Enterocooci count (TEC) were estimated by using plate count agar (PCA) and Bile Esculineagar plates respectively. Various Selective media and Biochemical tests were used for presumptive isolation and identification. Kirby-Bauer disc diffusion method on Mueller Hinton agar media was used for the determination of sensitivity of the isolated strains to commonly prescribe antibiotics. A total of 24 meat samples were examined of which 92% were positive to Enterococcus spp whereas 62.5% were Chicken and 32.5% pigeon. The counts of enterococci range of 0 - 6.49 log CFU/gm in chicken and 4.4 - 6.4 log CFU/gm in pigeon. 22 isolates of enterococci, 77.27% were resistance to Azithromycin, 54.55% to Nalidixic acid and 50% to Sulfamethoxazole. We found high sensitivity of antimicrobial agent against to Imipenem (100%), Ciprofloxacin (81.81%) and (77.27%) both Tetracycline and Erythromycin. Conclusion: Significance and Impact of the study: The study result expressed that awareness of the meat pathogen and when consumption of meat was well cooked of our daily life.