TITLE:
Evaluation of the Liver Cancer Prevention of Anthocyanin Extracts from Mulberry (Morus alba L.) Variety PR-01
AUTHORS:
Sufeng Liao, Jianghong Liu, Ming Xu, Jingui Zheng
KEYWORDS:
Mulberry (Morus alba L.) Anthocyanins Extracts, N-Nitrosodiethylamine, Hepatocarcinogenesis, Antioxidation, Anti-Inflammatory
JOURNAL NAME:
Advances in Bioscience and Biotechnology,
Vol.9 No.9,
September
18,
2018
ABSTRACT: This study aims to evaluate the preventive effects
of anthocyanins extracts (MAEs) from mulberry
variety PR-01 against N-nitrosodiethylamine (NDEA)-induced hepatocarcinogenesis in rats. It was found that 150 mg·kg-1 MAEs treatment significantly reduced the NDEA-induced
hepatic nodules incidence and hepatocellular carcinoma incidence by 58.30% and
41.70% compared to the model group. Meanwhile, MAEs significantly restored the
elevated the liver function enzymes, inhibited the tumor necrosis factor alpha
and interleukin-6 levels, elevated the serum interleukin-10 and interferon-γ and
increased hepatic glutathione-S-transferase and UDP-glucuronosyltransferase 2B1 enzyme activity. Moreover, 150 mg·kg-1 MAEs supplement enhanced glutathione content and
the activities of superoxide dismutase, catalase, glutathione peroxidase
activities but reduced the malondialdehyde and thiobarbituric acid-reactive
substances content by 37.90% and 44.52%. Furthermore, MAEs pretreatment maintained
nuclear factor erythroid 2-related factor 2 (Nrf2),
Kelch-like ECH-associated protein 1, heme oxygenase-1, and NAD(P)H: quinine
oxidoreductase1 stimulation and inhibited the expression of TNF-α, nuclear factor-kappaB (NF-κB), and cyclooxygenase-2 (COX-2), indicating
that MAEs exhibit effectively prevention effects against liver cancer via
decreased lipid peroxidation, induced Nrf2-mediated antioxidant enzymes and
attenuating the inflammatory mediators COX-2 through NF-κB pathway. Thus, MAEs of mulberry variety PR-01 may be used as a
good functional dietary supplement against liver cancer.