TITLE:
Analysis of Hyaluronidase Expression by qPCR in Egyptian Clinical Isolates of Staphylococcus aureus and Its Correlation with Phenotypic Plate Assay
AUTHORS:
Sarah A. Abdelkader, Mohammed El-Mowafy, Eman Abdelmegeed, Ramadan Hassan
KEYWORDS:
Hyaluronidase, qPCR, Staphylococcus aureus, Virulence Factor
JOURNAL NAME:
Advances in Microbiology,
Vol.8 No.7,
July
30,
2018
ABSTRACT: Hyaluronidase enzyme (HysA) is an extracellular enzyme that is considered to be an important virulence factor for Staphylococcus aureus. We screened the production of HysA enzyme in the spent media of Egyptian clinical isolates (32 isolates) via phenotypic plate assay. We found that 75% of the isolates (24 isolates) were able to produce HysA enzyme. We designed primers for qPCR analysis of hysA mRNA expression that was derived from the alignment of hysA gene sequences of 41 strains of S. aureus. The designed primers could be used for the amplification of hysA in 79.2% of the isolates (19 isolates) that were positive for HysA production as demonstrated by phenotypic plate assay. A significant positive correlation, as indicated by Pearson correlation analysis (r = 0.84 at P