TITLE:
Combinatorial Enzyme Approach to Produce Oligosaccharides of Diverse Structures for Functional Screen
AUTHORS:
Dominic W. S. Wong, Doris Feng, Sarah Batt, William Orts
KEYWORDS:
Combinatorial Enzyme, Polygalacturonase, Pectate Lyase, Pectin, Oligosaccharides
JOURNAL NAME:
Advances in Enzyme Research,
Vol.6 No.2,
June
29,
2018
ABSTRACT: Combinatorial chemistry has been a focus of research activity in modern drug discovery and biotechnology. It is a concept by which a vast library of molecular diversity is synthesized and screened for target properties. This report is to illustrate the application of enzyme technology using the concept of combinatorial chemistry as a novel approach for the bioconversion of plant fibers. Citrus pectin was subjected to combinatorial enzyme digestion to create libraries of pectic oligosaccharides with diverse structural variants. Repeated cycles of fractionation and screening resulted in the isolation and identification of an active oligoGalA species with antimicrobial activity.